This study explores the integration of Artificial Intelligence (AI) and Computer-Assisted Language Learning (CALL) in the professional development of in-service Venezuelan English teachers. Despite the growing use of AI in education, there is a need for further research on teachers’ training needs (Warschauer, M., & Xu, Y., 2024). This research examines teachers’ opinions on AI, their familiarity with AI and CALL tools, and their experiences with these technologies in the classroom. The survey results reveal teachers’ concerns about the effectiveness and challenges of AI and CALL in their teaching practices. The findings also highlight the need for support to help teachers integrate AI-based instruction. As Lee et al. (2024) emphasize, AI-based instruction is most effective when guided by teachers’ expertise and knowledge. This study contributes to the ongoing research on AI in education by providing insights into the perspectives and experiences of Venezuelan English teachers with AI and CALL technologies.